ytaF [2019-06-12 09:40:59]

[SW|sporulation] protein

locus: BSU29070

pI: 9.79

mw: 17.23 kDa

protein length: 210 aa

gene length: 633 bp

function: unknown

product: [SW|sporulation] protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,971,531 2,972,163

Phenotypes of a mutant

• strongly reduced [SW|sporulation] efficiency (10% as compared to wild type) [Pubmed|23396918]
• block in stage III of [SW|sporulation] (engulfment of the forespore) [Pubmed|23396918]

The protein

[SW|Domains]

• 4 potential transmembrane domains [Pubmed|23396918]

Expression and Regulation

Operons

• genes: [gene|3D6DA8B52A4CB49361E67906140696FFA1EF6099|polA]-[gene|EC96EDED2472607DFA4C5EBA054157F7832EE41C|mutM]-[gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]-[gene|E163A282560DB882B81E783E7E95E1960F4AE4D2|ytaG]
  description: [pubmed|22383849]

  sigma factors

  • [protein|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE]: sigma factor, [Pubmed|23396918], in [regulon|3CB24D493B3282278B7DC61CE3C793DFA815F9E2|SigE regulon]

Biological materials

Mutant

• MGNA-A117 (ytaF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/117 NBRP B. subtilis, Japan]
• BKE29070 ([gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE29070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC, downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT
• BKK29070 ([gene|3BEE8E248BC583FCCB37B7A209A122DD93CD398C|ytaF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK29070 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATCCGTTTCTTCCTTTC, downstream forward: _UP4_TAAAAAGGAGTGGATCTCTT

References

• 23396918